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by changing the focal point and after that three dimensional images are reconstructed by combining the images obtained from a confocal microscope .

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the significant amount of fluorescence emission that occurs at points above and below the objective focal plane is not confocal with the pinhole termed .

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the image was taken on a confocal microscope and the subsequent deconvolution was done using an experimentally derived point .

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confocal laser scanning microscopy clsm illuminates a single spot in the xy plane of a sample at a time emitted light is detected by a photomultiplier .

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confocal microscopy is an advanced light microscopy method which utilises a pinhole to eliminate out of focus light and is suitable for both live and .

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the image seen through a microscope includes the in focus portion and the out of focus portion above and below the plane of focus .

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